Electron Microscopy of the Sperm Tail Results Obtained with a New Fixative
نویسندگان
چکیده
منابع مشابه
Electron Microscopy of the Sperm Tail Results Obtained with a New Fixative
The details of a new fixation procedure using 40 per cent osmium tetroxide in carbon tetrachloride are presented. This fixative is a good general preservative, gives a higher contrast than the ordinary osmium fixatives, and may also preserve structures that are not otherwise readily revealed. Some possible reasons for the increased contrast are discussed. Micrographs of the sea urchin spermatoz...
متن کاملPermanganate—A New Fixative for Electron Microscopy
During the early phases of tissue sectioning for electron microscopy, virtually all of the standard cytological fixatives were tried. Of these, osmium tetroxide appeared to give the best preservation, and this was further improved by buffering the fixative solution at a slightly alkaline pH (Palade (1)). A variant developed by Dalton (2) contains Os04 in a chromate-dichromate system. Formalin h...
متن کاملLocalization of Adenosine Triphosphatase Activity in the Rat Sperm Tail as Revealed by Electron Microscopy
The epididymides of rat testis were fixed in glutaraldehyde and cut as frozen sections. The sections were incubated in lead nitrate solution containing as a substrate either ATP, AMP, creatinine phosphate, beta glycerophosphate, or phenyl phosphate. Then they were postfixed in osmium tetroxide, embedded, sectioned, and examined with the electron microscope. In the sperm tail, when ATP is used a...
متن کاملComparison of Aerobic Sporadic Bacilli Structure with Electron Microscopy
I. The existence of differences and similarities in the sur- 4. face features not only of different organisms or groups but also within given species has been demonstrated by a variety of techniques. 2. The different reactions of various organisms to the Gram stain might well be taken as one piece of evidence, the use of the electron microscop and associated preparative techniques (includi...
متن کاملSperm-egg Interactions of Limulus Polyphemus with Scanning Electron Microscopy
The primitive horseshoe crab, Limulus polyphemus, is an excellent species for studies of intial sperm-egg interactions (Shoger and Brown, 1970) . The gametes are easily obtained from mature animals, and fertilization and development can be demonstrated year around in the laboratory . Although the egg is comparatively large, quite opaque, and difficult to prepare for experiments involving embedd...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: The Journal of Biophysical and Biochemical Cytology
سال: 1959
ISSN: 1540-8140,0021-9525
DOI: 10.1083/jcb.5.2.269